期刊
CELL REPORTS
卷 29, 期 13, 页码 4422-+出版社
CELL PRESS
DOI: 10.1016/j.celrep.2019.11.108
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资金
- EMBO [ALTF 908-2017]
- Swiss National Science Foundation (ERC Transfer Grant [GermMethylation])
- Swiss National Science Foundation (Project Grant [PIWIorigins])
- Swiss National Science Foundation (NCCR RNA Disease)
- Republic of Geneva
- Canton of Geneva
RNA polymerase II transcripts receive a protective 5',5'-triphosphate-linked 7-methylguanosine (m(7)G) cap, and its removal by decapping enzymes like DCP2 is critical for initiation of RNA decay. Alternative RNA caps can be acquired when transcription initiation uses metabolites like nicotinamide adenine dinucleotide (NAD), generating NAD-RNAs. Here, we identify human NUDT12 as a cytosolic NAD-RNA decapping enzyme. NUDT12 is active only as homodimers, with each monomer contributing to creation of the two functional catalytic pockets. We identify an similar to 600-kDa dodecamer complex between bleomycin hydrolase (BLMH) and NUDT12, with BLMH being required for localization of NUDT12 to a few discrete cytoplasmic granules that are distinct from P-bodies. Both proteins downregulate gene expression when artificially tethered to a reporter RNA in vivo. Furthermore, loss of Nudt12 results in a significant upregulation of circadian clock transcripts in mouse liver. Overall, our study points to a physiological role for NUDT12 in the cytosolic surveillance of NAD-RNAs.
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