期刊
SCIENTIFIC REPORTS
卷 10, 期 1, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-020-58967-8
关键词
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资金
- CNRS, Universite de Toulouse-UPS
- Ibisa
- European structural funds
- Midi-Pyrenees region
- Agence Nationale de la Recherche (FASMY) [ANR-14-CE16-0012, ANR-10-LABX-62-IBEID, ANR-16-CE35-0009, ANR16-CE15-0003]
- MSDAVENIR (FIGHT-TB: Unconventional Strategies for Tuberculosis Treatment)
- Agence Nationale de la Recherche (ANR) [ANR-14-CE16-0012] Funding Source: Agence Nationale de la Recherche (ANR)
The fatty acid synthase type II (FAS-II) multienzyme system builds the main chain of mycolic acids (MAs), important lipid pathogenicity factors of Mycobacterium tuberculosis (Mtb). Due to their original structure, the identification of the (3 R)-hydroxyacyl-ACP dehydratases, HadAB and HadBC, of Mtb FAS-II complex required in-depth work. Here, we report the discovery of a third dehydratase protein, HadD(Mtb) (Rv0504c), whose gene is non-essential and sits upstream of cmaA2 encoding a cyclopropane synthase dedicated to keto- and methoxy-MAs. HadD(Mtb) deletion triggered a marked change in Mtb keto-MA content and size distribution, deeply impacting the production of full-size molecules. Furthermore, abnormal MAs, likely generated from 3-hydroxylated intermediates, accumulated. These data strongly suggest that HadD(Mtb) catalyzes the 3-hydroxyacyl dehydratation step of late FAS-II elongation cycles during keto-MA biosynthesis. Phenotyping of Mtb hadD deletion mutant revealed the influence of HadD(Mtb) on the planktonic growth, colony morphology and biofilm structuration, as well as on low temperature tolerance. Importantly, HadD(Mtb) has a strong impact on Mtb virulence in the mouse model of infection. The effects of the lack of HadD(Mtb) observed both in vitro and in vivo designate this protein as a bona fide target for the development of novel anti-TB intervention strategies.
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