期刊
SCIENTIFIC REPORTS
卷 9, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-019-54654-5
关键词
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资金
- Cooperative Research Project for Advanced Photonic Bioimaging, Ministry of Education, Culture, Sports, Science, and Technology (MEXT)/Japan Society for the Promotion of Science KAKENHI [15KT0072, 17K08561, 18H04927, 15K01831, 15K12763, 15H04679, 16K16645]
- Japan Science and Technology Agency PRESTO
- Nakayama Foundation for Human Science
- Research Foundation for Opto-Science and Technology
- Takeda Science Foundation
- Mochida memorial foundation
- Ichiro Kanehara Foundation
- Suhara Memorial Foundation
- Uehara Memorial Foundation
- Project for Developing Innovation Systems
- Research Program of Five-star Alliance in the Network Joint Research Center for Materials and Devices of MEXT
- Grants-in-Aid for Scientific Research [15K12763, 15KT0072, 15K01831, 18H04927, 17K08561, 15H04679, 16K16645] Funding Source: KAKEN
Circadian rhythms in Per1, PER2 expression and intracellular Ca2+ were measured from a solitary SCN neuron or glial cell which was physically isolated from other cells. Dispersed cells were cultured on a platform of microisland (100-200 mu m in diameter) in a culture dish. Significant circadian rhythms were detected in 57.1% for Per1 and 70.0% for PER2 expression. When two neurons were located on the same island, the circadian rhythms showed desynchronization, indicating a lack of oscillatory coupling. Circadian rhythms were also detected in intracellular Ca2+ of solitary SCN neurons. The ratio of circadian positive neurons was significantly larger without co-habitant of glial cells (84.4%) than with it (25.0%). A relatively large fraction of SCN neurons generates the intrinsic circadian oscillation without neural or humoral networks. In addition, glial cells seem to interrupt the expression of the circadian rhythmicity of intracellular Ca2+ under these conditions.
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