期刊
INTERFACE FOCUS
卷 10, 期 2, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rsfs.2019.0070
关键词
3D; tumour spheroid; microscopy; ImageJ; cell migration
类别
资金
- PPR Foundation [RGCALA106893-002]
- MRC [MR/K015613/1]
- MRC [MR/R025762/1, MR/K015613/1] Funding Source: UKRI
The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal-amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.
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