4.4 Article

A novel workflow for three-dimensional analysis of tumour cell migration

期刊

INTERFACE FOCUS
卷 10, 期 2, 页码 -

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ROYAL SOC
DOI: 10.1098/rsfs.2019.0070

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3D; tumour spheroid; microscopy; ImageJ; cell migration

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资金

  1. PPR Foundation [RGCALA106893-002]
  2. MRC [MR/K015613/1]
  3. MRC [MR/R025762/1, MR/K015613/1] Funding Source: UKRI

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The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal-amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.

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