期刊
HUMAN FERTILITY
卷 25, 期 1, 页码 135-141出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14647273.2019.1711204
关键词
Varicocele; microsurgical varicocelectomy; sperm telomeres; sperm DNA fragmentation male infertility; FISH
资金
- European Regional Development Fund (ERDF)
- Instituto de Salud Carlos III (Economy, Industry and Competitiveness Ministry, Madrid, Spain) [PI14/00119]
Varicocele is a major cause of male infertility, and microsurgical varicocelectomy appears to be an effective procedure for reducing testicular oxidative stress. However, it does not affect sperm telomere length and has only partial effects on seminal parameters and DNA damage in varicocele patients.
Varicocele is one of the main causes of male infertility and microsurgical varicocelectomy (MV) seems to be the best procedure for its repair and to reduce testicular oxidative stress (ROS). As ROS causes guanine modifications, we postulated that DNA damage could be more intense in telomeres due to their G-rich nature. We studied the effect of MV on sperm telomere length (TL), single- and double-strand DNA fragmentation (ssSDF and dsSDF) and seminal parameters. Sperm telomeres from 12 fertile donors and 20 varicocele patients before and nine months after MV were labelled using FITC-PNA qFISH (a new method to obtain absolute TL from relative fluorescence intensity using FITC-fluorescent spheres). Both ssSDF and dsSDF were analysed using the alkaline and neutral Comet assays, respectively. The results showed that varicocele and MV had no effect on TL. Seminal parameters, ssSDF and dsSDF of varicocele patients were altered. Although these parameters improved after MV, values did not reach those seen in fertile donors. A good estimation of absolute TL was developed based on FITC-fluorescent spheres. The results showed that TL is not affected by varicocele or surgery. However, MV is able to partially reduce altered seminal parameters, ssSDF and dsSDF values in varicocele patients.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据