期刊
DRUG DESIGN DEVELOPMENT AND THERAPY
卷 14, 期 -, 页码 527-538出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/DDDT.S235491
关键词
Panax notoginseng; diabetic nephropathy; podocyte; Wnt/beta-catenin; epithelial-mesenchymal transition
资金
- National Natural Science Foundation of China [81774052, 81573738]
- Pudong New Area Science and Technology Commission [PKJ2015-Y10]
- Shanghai Pujiang Program [17PJD031]
- Three-year action plan for Shanghai further accelerating the development of traditional Chinese medicine [ZY (2018-2020)-CCCX-4007]
Introduction: Epithelial-mesenchymal transition (EMT) may contribute to podocyte dysfunction in diabetic nephropathy (DN). Aiming to identify novel therapeutic options, we investigated the protective effects of Panax notoginseng (PN) on podocyte EMT in diabetic rats and explored its mechanisms. Methods: Diabetes was induced in rats with streptozotocin (STZ) by intraperitoneal injection at 55 mg/kg. Diabetic rats were randomly divided into three groups, namely, diabetic rats, diabetic rats treated with beraprost sodium (BPS) at 0.6 mg/kg/d or PN at 0.4 g/kg/d p. o., for 12 weeks. Urinary albumin/creatinine ratio (ACR), biochemical parameters, renal histopathology, and podocyte morphological changes were evaluated. Protein expression of EMT markers (desmin, alpha-SMA, and nephrin) as well as components of the Wnt/beta-catenin pathway (wntl, beta-catenin, and snail) was detected by immunohistochemistry and Western blot, respectively. Results: In diabetic rats, severe hyperglycemia and albuminuria were detected. Moreover, mesangial expansion and podocyte foot process effacement were found markedly increased in diabetic kidneys. Increased protein expression of wnt1, beta-catenin, snail, desmin, and alpha-SMA, as well as decreased protein expression of nephrin was detected in diabetic kidneys. All these abnormalities found in DN rats were partially restored by PN treatment. Conclusion: PN ameliorated albuminuria and podocyte EMT in diabetic rats partly through inhibiting Wnt/beta-catenin signaling pathway. These findings provide experimental arguments for a novel therapeutic option in DN.
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