Polarimetric second-harmonic generation microscopy of the hierarchical structure of collagen in stage I-III non-small cell lung carcinoma

Polarimetric second-harmonic generation (P-SHG) microscopy is used to quantify the structural alteration of collagen in stage-I,-II and -III non-small cell lung carcinoma (NSCLC) ex vivo tissue. The achiral and chiral molecular second-order susceptibility tensor components ratios (R and C, respectively), the degree of linear polarization (DLP) and the in-plane collagen fiber orientation (delta) were extracted. Further, texture analysis was performed on the SHG intensity, R, C, DLP and delta. The distributions of R, C, DLP and delta as well as the textural features of entropy, correlation and contrast show significant differences between normal and tumor tissues. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement