期刊
NATURE COMMUNICATIONS
卷 11, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-020-14936-3
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资金
- Swedish Research Council
- Swedish Research Council for Sustainable Development
- Swedish Childhood Cancer Foundation
- Karolinska Institutet
- Knut and Alice Wallenberg Foundation
- Wallenberg Academy Fellow
- Ragnar Soderberg Foundation
- The Ming Wai Lau Center for Reparative Medicine, Center for Innovative Medicine/SLL
- Scandinavia-Japan Sasakawa Foundation
- Japan Eye Bank Association
- Astellas Foundation for Research on Metabolic Disorders
- Japan Society for the Promotion of Science Overseas Research Fellowships
- European Union [825100]
- Karolinska Institute
- H2020 Societal Challenges Programme [825100] Funding Source: H2020 Societal Challenges Programme
The human ovary orchestrates sex hormone production and undergoes monthly structural changes to release mature oocytes. The outer lining of the ovary (cortex) has a key role in defining fertility in women as it harbors the ovarian reserve. It has been postulated that putative oogonial stem cells exist in the ovarian cortex and that these can be captured by DDX4 antibody isolation. Here, we report single-cell transcriptomes and cell surface antigen profiles of over 24,000 cells from high quality ovarian cortex samples from 21 patients. Our data identify transcriptional profiles of six main cell types; oocytes, granulosa cells, immune cells, endothelial cells, perivascular cells, and stromal cells. Cells captured by DDX4 antibody are perivascular cells, not oogonial stem cells. Our data do not support the existence of germline stem cells in adult human ovaries, thereby reinforcing the dogma of a limited ovarian reserve. The outer lining or cortex of the human ovary determines fertility and has been postulated to contain oogonial stem cells. Here, the authors generate a single-cell map of the adult human ovarian cortex and show that DDX4 labels perivascular cells but no oogonial stem cells.
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