期刊
NATURE COMMUNICATIONS
卷 11, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-019-14012-5
关键词
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资金
- ONR [N00014-17-1-2353]
- NSF [MCB-1453219]
- NIH [1R01AI132403-01]
- Carlsberg Foundation
- Fannie and John Hertz Foundation Fellowship
- NSF Graduate Research Fellowship [DGE-1644869]
Horizontal gene transfer (HGT) in complex bacterial communities has been mostly studied using metagenomic analyses. Here, the authors develop an E. coli CRISPR-Cas spacer acquisition platform that allows real-time recording of HGT events at nucleotide-resolution, identifying diverse DNA transfer events in human clinical fecal samples. The flow of genetic material between bacteria is central to the adaptation and evolution of bacterial genomes. However, our knowledge about DNA transfer within complex microbiomes is lacking, with most studies of horizontal gene transfer (HGT) relying on bioinformatic analyses of genetic elements maintained on evolutionary timescales or experimental measurements of phenotypically trackable markers. Here, we utilize the CRISPR-Cas spacer acquisition process to detect DNA acquisition events from complex microbiota in real-time and at nucleotide resolution. In this system, an E. coli recording strain is exposed to a microbial sample and spacers are acquired from transferred plasmids and permanently stored in genomic CRISPR arrays. Sequencing and analysis of acquired spacers enables identification of the transferred plasmids. This approach allowed us to identify individual mobile elements without relying on phenotypic markers or post-transfer replication. We found that HGT into the recording strain in human clinical fecal samples can be extensive and is driven by different plasmid types, with the IncX type being the most actively transferred.
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