Photobactericidal activity activated by thiolated gold nanoclusters at low flux levels of white light

The emergence of antibiotic resistant bacteria is a major threat to the practice of modern medicine. Photobactericidal agents have obtained significant attention as promising candidates to kill bacteria, and they have been extensively studied. However, to obtain photobactericidal activity, an intense white light source or UV-activation is usually required. Here we report a photobactericidal polymer containing crystal violet (CV) and thiolated gold nanocluster ([Au-25(Cys)(18)]) activated at a low flux levels of white light. It was shown that the polymer encapsulated with CV do not have photobactericidal activity under white light illumination of an average 312 lux. However, encapsulation of [Au-25(Cys)(18)] and CV into the polymer activates potent photobactericidal activity. The study of the photobactericidal mechanism shows that additional encapsulation of [Au-25(Cys)(18)] into the CV treated polymer promotes redox reactions through generation of alternative electron transfer pathways, while it reduces photochemical reaction type- pathways resulting in promotion of hydrogen peroxide (H2O2) production. Antibiotic resistant bacteria have increased the interest in the development of antibacterial surfaces to reduce the risk of infection. Here, the authors report on a thiolated gold nanocluster enhanced crystal violet polymer with bactericidal properties under low level white light illumination.