4.8 Article

Enhancing neuronal chloride extrusion rescues alpha 2/alpha 3 GABA(A)-mediated analgesia in neuropathic pain

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NATURE COMMUNICATIONS
卷 11, 期 1, 页码 -

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NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-019-14154-6

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资金

  1. CIHR [MOP-12942, MOP-79411, MOP-136903]
  2. NSERC [109166, 06507]
  3. Canada Research Chair in Chronic Pain and Related Brain Disorders
  4. Chercheur-Boursier Award from the Fonds de recherche en sante du Quebec
  5. People Program Marie Curie Actions grant of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA grant agreement [318 997 - NEUREN]

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Spinal disinhibition has been hypothesized to underlie pain hypersensitivity in neuropathic pain. Apparently contradictory mechanisms have been reported, raising questions on the best target to produce analgesia. Here, we show that nerve injury is associated with a reduction in the number of inhibitory synapses in the spinal dorsal horn. Paradoxically, this is accompanied by a BDNF-TrkB-mediated upregulation of synaptic GABA(A)Rs and by an alpha 1-to-alpha 2GABA(A)R subunit switch, providing a mechanistic rationale for the analgesic action of the alpha 2,3GABA(A)R benzodiazepine-site ligand L838,417 after nerve injury. Yet, we demonstrate that impaired Cl- extrusion underlies the failure of L838,417 to induce analgesia at high doses due to a resulting collapse in Cl- gradient, dramatically limiting the benzodiazepine therapeutic window. In turn, enhancing KCC2 activity not only potentiated L838,417-induced analgesia, it rescued its analgesic potential at high doses, revealing a novel strategy for analgesia in pathological pain, by combined targeting of the appropriate GABA(A)R-subtypes and restoring Cl- homeostasis.

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