4.8 Article

Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain

期刊

NATURE COMMUNICATIONS
卷 11, 期 1, 页码 -

出版社

NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-020-14350-9

关键词

-

资金

  1. European Union's Horizon 2020 research and innovation programme [647784]
  2. U.S. National Institute of General Medical Sciences [GM19559, GM131705]
  3. U.S. National Institutes of Health [P41GM104601]
  4. U.S. National Science Foundation [PHY1430124]
  5. U.K. Biotechnology and Biological Sciences Research Council [BB/S003339/1]
  6. Wellcome [208361/Z/17/Z]
  7. BBSRC [BB/P01948X/1, BB/R002517/1]
  8. MRC [MR/S009213/1]
  9. EPSRC [EP/L000253/1]
  10. Wellcome Trust
  11. iNEXT - EU Horizon 2020 programme [653706, PID:2626]
  12. FRISBI [ANR-10-INSB-05-02]
  13. GRAL, a project of the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBHEUR-GS [ANR-17-EURE-0003]
  14. Rhone-Alpes Region
  15. Fondation pour la Recherche Medicale (FRM)
  16. fonds FEDER
  17. Centre National de la Recherche Scientifique (CNRS)
  18. Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA)
  19. University of Grenoble Alpes
  20. EMBL
  21. GIS-Infrastructures en Biologie Sante et Agronomie (IBISA)
  22. National Science Foundation [OCI-0725070, ACI-1238993]
  23. state of Illinois as part of the Petascale Computational Resource Grant [ACI-1713784]
  24. BBSRC [BB/P01948X/1, BB/S003339/1, BB/R002517/1, BB/P01948X/2] Funding Source: UKRI
  25. EPSRC [EP/R029407/1] Funding Source: UKRI
  26. European Research Council (ERC) [647784] Funding Source: European Research Council (ERC)

向作者/读者索取更多资源

Motile bacteria sense chemical gradients with transmembrane receptors organised in supramolecular signalling arrays. Understanding stimulus detection and transmission at the molecular level requires precise structural characterisation of the array building block known as a core signalling unit. Here we introduce an Escherichia coli strain that forms small minicells possessing extended and highly ordered chemosensory arrays. We use cryo-electron tomography and subtomogram averaging to provide a three-dimensional map of a complete core signalling unit, with visible densities corresponding to the HAMP and periplasmic domains. This map, combined with previously determined high resolution structures and molecular dynamics simulations, yields a molecular model of the transmembrane core signalling unit and enables spatial localisation of its individual domains. Our work thus offers a solid structural basis for the interpretation of a wide range of existing data and the design of further experiments to elucidate signalling mechanisms within the core signalling unit and larger array.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据