期刊
NATURE COMMUNICATIONS
卷 11, 期 1, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-020-14350-9
关键词
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资金
- European Union's Horizon 2020 research and innovation programme [647784]
- U.S. National Institute of General Medical Sciences [GM19559, GM131705]
- U.S. National Institutes of Health [P41GM104601]
- U.S. National Science Foundation [PHY1430124]
- U.K. Biotechnology and Biological Sciences Research Council [BB/S003339/1]
- Wellcome [208361/Z/17/Z]
- BBSRC [BB/P01948X/1, BB/R002517/1]
- MRC [MR/S009213/1]
- EPSRC [EP/L000253/1]
- Wellcome Trust
- iNEXT - EU Horizon 2020 programme [653706, PID:2626]
- FRISBI [ANR-10-INSB-05-02]
- GRAL, a project of the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBHEUR-GS [ANR-17-EURE-0003]
- Rhone-Alpes Region
- Fondation pour la Recherche Medicale (FRM)
- fonds FEDER
- Centre National de la Recherche Scientifique (CNRS)
- Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA)
- University of Grenoble Alpes
- EMBL
- GIS-Infrastructures en Biologie Sante et Agronomie (IBISA)
- National Science Foundation [OCI-0725070, ACI-1238993]
- state of Illinois as part of the Petascale Computational Resource Grant [ACI-1713784]
- BBSRC [BB/P01948X/1, BB/S003339/1, BB/R002517/1, BB/P01948X/2] Funding Source: UKRI
- EPSRC [EP/R029407/1] Funding Source: UKRI
- European Research Council (ERC) [647784] Funding Source: European Research Council (ERC)
Motile bacteria sense chemical gradients with transmembrane receptors organised in supramolecular signalling arrays. Understanding stimulus detection and transmission at the molecular level requires precise structural characterisation of the array building block known as a core signalling unit. Here we introduce an Escherichia coli strain that forms small minicells possessing extended and highly ordered chemosensory arrays. We use cryo-electron tomography and subtomogram averaging to provide a three-dimensional map of a complete core signalling unit, with visible densities corresponding to the HAMP and periplasmic domains. This map, combined with previously determined high resolution structures and molecular dynamics simulations, yields a molecular model of the transmembrane core signalling unit and enables spatial localisation of its individual domains. Our work thus offers a solid structural basis for the interpretation of a wide range of existing data and the design of further experiments to elucidate signalling mechanisms within the core signalling unit and larger array.
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