期刊
NATURE COMMUNICATIONS
卷 10, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-019-13605-4
关键词
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资金
- Horizon 2020 grant [INTENS 668294]
- OAK Foundation [W1095/OCAY-14-191]
- NIHR GOSH BRC Catalyst Fellowship
- GOSHCC Studentship [V6116]
- Netherlands Organisation for Scientific Research VENI NWO-ZonMW [016.166.140]
- Human Frontier Science Program Organization [HFSPO LT771/2015]
- Francis Crick Institute
- Cancer Research UK
- UK Medical Research Council
- Wellcome Trust [FC001105]
- Rosetrees Trust [M362-F1]
- University of Padova
- NIHR [NIHR-RP-2014-04-046]
- NIHR GOSH BRC
- ShanghaiTech University
- [F-0301-15-009]
Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.
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