期刊
ONCOLOGY LETTERS
卷 19, 期 4, 页码 3215-3222出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2020.11413
关键词
advanced glycation end products; proliferation; invasion; epithelial-mesenchymal transition; PI3K; AKT
类别
资金
- Key Projects of Guangxi Natural Science Foundation [2013GXNSFDA019019]
- '139' plan for Training High Level Cadre Talents in Guangxi Medicine
- Guangxi Special Fund Project for Cultivating Academic and Technical Leaders in the New Century
- Second Level in Guangxi New Century Ten, Hundred and Thousand Talents Project
The aim of the present study was to determine the mechanism by which advanced glycation end products (AGEs) induce proliferation, invasion and epithelial-mesenchymal transition (EMT) of human colon cancer SW480 cells. SW480 cells were divided into groups as follows: i) Control; ii) cells treated with AGEs alone; and iii) cells treated with AGEs combined with LY294002. Proliferation, cell cycle progression, apoptosis, invasion and migration of SW480 cells were assessed using an MTT assay, flow cytometry, Transwell assays and a wound healing assay, respectively. The protein expression levels of PI3K, AKT and epithelial cadherin (E-cadherin) were examined by western blot analysis in SW480 cells treated with various concentrations of AGEs. Proliferation, invasion and migration were enhanced, cell cycle progression was increased and apoptosis was decreased in SW480 cells treated with AGEs compared with the control. The PI3K inhibitor, LY294002, reversed the effects of AGEs. Western blot analysis data demonstrated that AGEs increased the protein expression levels of PI3K and AKT, and decreased the expression of E-cadherin. The results suggested that AGEs exert a positive effect on the proliferation, invasion and EMT in SW480 cells through the PI3K/AKT signaling pathway.
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