期刊
BIOSENSORS & BIOELECTRONICS
卷 64, 期 -, 页码 165-170出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.08.078
关键词
Apoferritin; Gold clusters; Peroxidase-like activity; Kinetic study; Glucose
类别
资金
- National Natural Science Foundation of China [81271697]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20100061120077, 20120061110021]
- Social Development Project of Science and Technology Department of Jilin Province, China [20106031, 20120967, YYZX201264, 20130206069GX]
- Fundamental Research Funds for the Central Universities
- Significant New Drug Creation Science and Technology Major Program [2012ZX09503001-003]
The discovery and application of noble metal nanoclusters have received considerable attention. In this paper, we reported that apoferritin paired gold clusters (Au-Ft) could efficiently catalyze oxidation of 3.3',5.5'-tetramethylbenzidine (TMB) by H2O2 to produce a blue color reaction. Compared with natural enzyme, Au-Ft exhibited higher activity near acidic pH and could be used over a wide range of temperatures. Apoferritin nanocage enhanced the reaction activity of substrate TMB by H2O2. The reaction catalyzed by Au-Ft was found to follow a typical Michaelis-Menten kinetics. The kinetic parameters exhibited a lower K-m value (0.097 mM) and a higher K-cat value (5.8 x 10(4) s(-1)) for TMB than that of horse radish peroxidase (HRP). Base on these findings, Au-Ft, acting as a peroxidase mimetic, performed enzymatic spectrophotometric analysis of glucose. This system exhibited acceptable reproducibility and high selectivity in biosening, suggesting that it could have promising applications in the future. (C) 2014 Elsevier B.V. All rights reserved.
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