期刊
VIRUSES-BASEL
卷 12, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/v12020192
关键词
bacteriophage; endolysin; thermostable lytic protein; antibiotic-resistant bacteria; antimicrobial drug; Staphylococcus aureus; skin infection
类别
资金
- Natural Science Foundation of China [31760042, 31760338]
- Project of Innovation and Entrepreneurship Training for College Students of Yunnan Province in 2019 [201910674243]
- Kunming University of Science and Technology for the Introduction of Talent Research Project [KKSY201626003]
New strategies against antibiotic-resistant bacterial pathogens are urgently needed but are not within reach. Here, we present in vitro and in vivo antimicrobial activity of TSPphg, a novel phage lysin identified from extremophilic Thermus phage TSP4 by sequencing its whole genome. By breaking down the bacterial cells, TSPphg is able to cause bacteria destruction and has shown bactericidal activity against both Gram-negative and Gram-positive pathogenic bacteria, especially antibiotic-resistant strains of Klebsiella pneumoniae, in which the complete elimination and highest reduction in bacterial counts by greater than 6 logs were observed upon 50 mu g/mL TSPphg treatment at 37 degrees C for 1 h. A murine skin infection model further confirmed the in vivo efficacy of TSPphg in removing a highly dangerous and multidrug-resistant Staphylococcus aureus from skin damage and in accelerating wound closure. Together, our findings may offer a therapeutic alternative to help fight bacterial infections in the current age of mounting antibiotic resistance, and to shed light on bacteriophage-based strategies to develop novel anti-infectives.
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