期刊
BIOSENSORS & BIOELECTRONICS
卷 74, 期 -, 页码 134-141出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.05.025
关键词
Nanozyme; Immunochromatographic strip; Ebola detection
类别
资金
- National Science and Technology Major Project [2013ZX10004102, 2012ZX10002009-016]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA09030306]
- National Natural Science Foundation of China [31270908, 81201698]
- 973 Program [2011CB933503, 2012CB934003]
- Knowledge Innovation Program of the Chinese Academy of Sciences [CXJJ-14-M24]
- special project of Ebola virus research from the president foundation of Chinese Academy of Sciences
Ebola continues to rage in West Africa. In the absence of an approved vaccine or treatment, the priority in controlling this epidemic is to promptly identify and isolate infected individuals. To this end, a rapid, highly sensitive, and easy-to-use test for Ebola diagnosis is urgently needed. Here, by using Fe3O4 magnetic nanoparticle (MNP) as a nanozyme probe, we developed a MNP-based immunochromatographic strip (Nanozyme-strip), which detects the glycoprotein of Ebola virus (EBOV) as low as 1 ng/mL, which is 100-fold more sensitive than the standard strip method. The sensitivity of the Nanozyme-strip for EBOV detection and diagnostic accuracy for New Bunyavirus clinical samples is comparable with ELISA, but is much faster (within 30 min) and simpler (without need of specialist facilities). The results demonstrate that the Nanozyme-strip test can rapidly and sensitively detect EBOV, providing a valuable simple screening tool for diagnosis of infection in Ebola-stricken areas. (C) 2015 Elsevier B.V. All rights reserved.
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