期刊
BIOSENSORS & BIOELECTRONICS
卷 68, 期 -, 页码 168-174出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.12.046
关键词
Upconversion nanoparticles; Gold nanoparticle; Fluorescence resonance energy transfer; Organophosphorus pesticides
类别
资金
- National Natural Science Foundation of China [21375037, 21275051, 21475043]
- Scientific Research Fund of Hunan Provincial Science and Technology Department and Education Department [13JJ2020, 12A084]
- Doctoral Fund of Ministry of Education of China [20134306110006]
This paper reports a novel nanosensor for organophosphorus pesticides based on the fluorescence resonance energy transfer (FRET) between NaYF4:Yb,Er upconversion nanoparticles (UCNPs) and gold nanoparticles (AuNPs). The detection mechanism is based on the facts that AuNPs quench the fluorescence of UCNPs and organophosphorus pesticides (OPs) inhibit the activity of acetylcholinesterase (AChE) which catalyzes the hydrolysis of acetylthiocholine (ATC) into thiocholine. Under the optimized conditions, the logarithm of the pesticides concentration was proportional to the inhibition efficiency. The detection limits of parathion-methyl, monocrotophos and dimethoate reached 0.67, 23, and 67 ng/L, respectively. Meanwhile, the biosensor shows good sensitivity, stability, and could be successfully applied to detection of OPs in real food samples, suggesting the biosensor has potentially extensive application clinic diagnoses assays. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据