期刊
BIOSENSORS & BIOELECTRONICS
卷 69, 期 -, 页码 1-7出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.01.011
关键词
Protein tyrosine nitration; Label-free in-situ monitoring; Surface-enhanced Raman spectroscopy
类别
资金
- National Basic Research 973 Program [2013CB733700]
- National Natural Science Foundation of China [21421004, 21125522, 21327807]
- Shanghai Pujiang Program Grant of China [12JC1403500]
- Shanghai Municipal Natural Science Foundation, China [14ZR1410800]
- Fundamental Research Funds for the Central Universities [WB1113005]
A novel label-free method for the in-situ monitoring of protein tyrosine nitration (PTN) was explored based on surface-enhanced Raman spectroscopy (SERS). Benefiting from the relative weak binding ability of sulfate to silver surface, the Raman signals of nitrated peptides were boosted well with sulfate-aggregated silver nanoparticles (Ag NPs). The distinction of the SERS spectra between non-nitrated peptides and nitrated peptides was obtained by directly comparing SERS bands at 330-400 cm(-1), allowing the rapid identification of PTN. Furthermore, without any pretreatments, the established method was successfully applied in the rapid in-situ dynamic monitoring of the mimic hemin-catalyzed PTN process in synthetic peptide, bovine serum albumin (BSA), and original human blood serum samples. The results indicated that the proposed approach could be a promising in-situ label-free tool for observing PTN process, which may be quite helpful to deeply understand the mechanism of post-translation modification. (C) 2015 Elsevier B.V. All rights reserved.
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