期刊
CANCER LETTERS
卷 380, 期 1, 页码 47-58出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2016.06.009
关键词
NCAM; FGF-2; FGFR1; Esophageal cancer; Macrophage; Tumor microenvironment
类别
资金
- Japan Society for the Promotion of Science [C-23590397, C-26460418, B-26870364]
Tumor-associated macrophages (TAMs) have important roles in the angiogenesis and tumor immuno-suppression of various cancers, including esophageal squamous cell carcinomas (ESCCs). To elucidate the roles of TAMs in ESCCs, we compared the gene expression profiles between human peripheral blood monocyte-derived macrophage-like cells (Macrophage_Ls) and Macrophage_Ls stimulated with conditioned medium of the TE series human ESCC cell line (TECM) (TAM_Ls) using cDNA microarray analysis. Among the highly expressed genes in TAM_Ls, we focused on neural cell adhesion molecule (NCAM). NCAM knockdown in TAM_Ls revealed a significant decrease of migration and survival via a suppression of PI3K-Akt and fibroblast growth factor receptor I (FGFR1) signaling. Stimulation by TECM up-regulated the level of FGFR1 in Macrophage_Ls. Recombinant human fibroblast growth factor-2 (rhFGF-2) promoted the migration and survival of TAM_Ls and TE-cells through FGFR1 signaling. Our immunohistochemical analysis of 70 surgically resected ESCC samples revealed that the up-regulated FGF-2 in stromal cells, including macrophages, was associated with more aggressive phenotypes and a high number of infiltrating M2 macrophages. These findings may indicate a novel role of NCAM- and FGF-2-mediated FGFRI signaling in the tumor microenvironment of ESCCs. (C) 2016 The Authors. Published by Elsevier Ireland Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据