期刊
ULTRAMICROSCOPY
卷 207, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ultramic.2019.112834
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类别
资金
- Gordon and Betty Moore foundation
- Stanford Graduate Fellowship
- National Science Foundation [1656518]
- Direct For Education and Human Resources
- Division Of Graduate Education [1656518] Funding Source: National Science Foundation
Multi-pass transmission electron microscopy (MPTEM) has been proposed as a way to reduce damage to radiation-sensitive materials. For the field of cryo-electron microscopy (cryo-EM), this would significantly reduce the number of projections needed to create a 3D model and would allow the imaging of lower-contrast, more heterogeneous samples. We have designed a 10 keV proof-of-concept MPTEM. The column features fast-switching gated electron mirrors which cause each electron to interrogate the sample multiple times. A linear approximation for the mull-pass contrast transfer function (CTF) is developed to explain how the resolution depends on the number of passes through the sample.
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