期刊
BIOSENSORS & BIOELECTRONICS
卷 71, 期 -, 页码 380-386出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.04.080
关键词
Circulating tumor cells; Microfluidic chip; PDMS-based filter; Sacrificial film
类别
资金
- 973 Program of the Ministry of Science and Technology of the People's Republic of China [2012CB933303, 2011CB707505]
- National Natural Science Foundation of China [61271161, 61271162, 21405167, 21407016, 81472751]
- CAS Scientific Research Equipment Development Program [YZ201337]
- Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University
Isolation of circulating tumor cells (CTCs) by size exclusion is a widely researched technique that offers the advantage of capturing tumor cells without reliance on cell surface expression markers. In this work, we report the development of a novel polydimethylsiloxane (PDMS) membrane filter-based microdevice for rapid and highly efficient isolation of CFCs from peripheral blood. A precise and highly porous PDMS microfilter was fabricated and integrated into the microfiltration chip by combining a sacrificial transferring film with a sandwich molding method. We achieved > 90% recovery when isolating lung cancer cells from spiked blood samples, with a relatively high processing throughput of 10 mL/h. In contrast to existing CTC filtration systems, which rely on low-porosity track-etch filters or expensive lithography-based filters, our microfiltration chip does not require complex e-beam lithography or the reactive ion etching process, therefore it offers a low-cost alternative tool for highly efficient CTC enrichment and in situ analysis. Thus, this new microdevice has the potential for use in routine monitoring of cancer development and cancer therapy in a clinical setting. (C) 2015 Elsevier B.V. All rights reserved.
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