期刊
TOXICOLOGY LETTERS
卷 321, 期 -, 页码 73-82出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2019.12.020
关键词
Methamphetamine; Addiction; Intestinal mucosal barrier; microRNA; TNF-alpha; Tight junction
类别
资金
- National Natural Science Foundation of China [3171101074, 81860100, 31860306, 81870458]
- Science and Technology Department of Yunnan Province [2018DH006, 2018NS0086]
- [YLXL20170002]
An enterogenic infection occurs when intestinal mucosal disruption is followed by the invasion of intestinal bacteria into the blood and distant organs, which can result in severe diseases or even death. Our previous study using Rhesus monkeys as an in vivo model revealed that methamphetamine (MA) induced intestinal mucosal barrier damage, which poses a high risk of enterogenic infection. However, how methamphetamine causes intestinal mucosal barrier damage remains largely unknown. In this study, we employed an in vitro model, and found that MA treatment could inhibit the expression of miR-181c, which directly targets and regulates TNF-alpha, and ultimately induces apoptosis and damages the intestinal barrier. Moreover, we measured TNF-alpha serum levels as well as the intestinal mucosal barrier damage indicators (diamine oxidase, D-lactic acid, and exotoxin) and found that their levels were significantly higher in MA-dependents than in healthy controls (P < 0.001). To the best of our knowledge, this is the first report evidencing that miR-181c is involved in MA-induced intestinal barrier injury via TNF-alpha regulation, which introduces novel potential therapeutic targets for MA-dependent intestinal diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据