期刊
BIOSENSORS & BIOELECTRONICS
卷 71, 期 -, 页码 269-277出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.04.044
关键词
Single-walled carbon nanotubes (SWCNTs); Cell-based sensing; Real-time analysis; Cell adhesion; Viability
类别
资金
- National Science Foundation (NSF) [EPS-1003907, 1434503]
- National Institute of Health (NIH) [R01-ES022968]
- West Virginia University (WVU)
Single-walled carbon nanotubes (SWCNTs) implementation in a variety of biomedical applications from bioimaging, to controlled drug delivery and cellular-directed alignment for muscle myofiber fabrication, has raised awareness of their potential toxicity. Nanotubes structural aspects which resemble asbestos, as well as their ability to induce cyto and genotoxicity upon interaction with biological systems by generating reactive oxygen species or inducing membrane damage, just to name a few, have led to focused efforts aimed to assess associated risks prior their user implementation. In this study, we employed a non-invasive and real-time electric cell impedance sensing (ECIS) platform to monitor behavior of lung epithelial cells upon exposure to a library of SWCNTs with user-defined physico-chemical properties. Using the natural sensitivity of the cells, we evaluated SWCNT-induced cellular changes in relation to cell attachment, cell-cell interactions and cell viability respectively. Our methods have the potential to lead to the development of standardized assays for risk assessment of other nanomaterials as well as risk differentiation based on the nanomaterials surface chemistry, purity and agglomeration state. (C) 2015 Elsevier B.V. All rights reserved.
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