期刊
STEM CELL RESEARCH
卷 41, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scr.2019.101654
关键词
-
资金
- Netherlands Organ-on-Chip Initiative, an NOW Gravitation project - Ministry of Education, Culture and Science of the government of the Netherlands [024.003.001]
Lymphoblast cells from four individuals of a family of two genetically unrelated parents and their monozygotic twins were used to generate integration-free human induced pluripotent stem cells (hiPSCs). Reprogramming factors were delivered by co-electroporation of three episomal-based plasmids expressing OCT3/4, SOX2, KLF4, L-MYC and LIN28. The hiPSCs showed a normal karyotype, expressed pluripotency-associated markers, displayed the capacity for in vitro differentiation into the three germ layers and were Epstein Barr virus-free. These hiPSC lines offer the possibility to compare genetically unrelated and genetically identical tissues from different individuals and to study genotype-specific effects, which are particularly relevant for toxicology testing.
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