4.7 Article

A simple and programmed DNA tweezer probes for one-step and amplified detection of UO22+

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2019.118017

关键词

Fluorescence; DNAzyme; Gold nanoparticles; DNA tweezer; One-step

资金

  1. Program of Innovation Center of Lipid Resources and Children's Daily Chemicals at Chongqing University of Education [2017XJPT01]
  2. China Postdoctoral Science Foundation [2019M653475]
  3. National Natural Science Foundation of China [31300819]
  4. Science and Technology Research Program of Chongqing Municipal Education Commission [KJQN201900842]
  5. Science and Technology Innovation Project of Chongqing Science and Technology Commission [cstc2017shms-zdyfX0063, cstc2019jscx-msxmX0334]

向作者/读者索取更多资源

A simple DNA tweezer was proposed for one-step and amplified detection of UO22+ based on DNAzyme catalytic cleavage. The two arms of DNA tweezers are close in the original form. Thus, the fluorescent signal of fluorophore at the end of arm is dramatically quenched. However, the structure of DNA tweezers can be changed from close to open in the presence of UO22+, resulting the strong fluorescent signal. The linear range was obtained in the range of 0.1 nM to 60 nM and the limit of detection was 25 pM with the amplification of DNAzyme catalytic cleavage reaction. Importantly, the whole detection process is very simple and only one operation step is required. In addition, it shows great potential and promising prospects for uranyl detection in practical application. (C) 2019 Published by Elsevier B.V.

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