期刊
SMALL
卷 16, 期 10, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201904064
关键词
anti-inflammation; exosome; extracellular vesicles; inflammation; lipoaspirate; microvesicle
类别
资金
- Mayo Clinic
- Center for Regenerative Medicine
- Mayo Clinic Florida Focused Research Team Program
- Mayo Clinic Investigator Award
- Mayo Professorship Award
- China Scholarship Council
- Muller-Stiftung Mannheim
- Vetter-Stiftung Mannheim
- State of Florida Cancer Award
- Jack & Peggy Sturm Foundation
- United States Department of Defense (DOD) [W81XWH-15-1-0257]
- United States National Cancer Institute [R01CA183827, R01CA195503]
- Technion Russell Berrie Nanotechnology Institute (RBNI)
Extracellular vesicles secreted from adipose-derived mesenchymal stem cells (ADSCs) have therapeutic effects in inflammatory diseases. However, production of extracellular vesicles (EVs) from ADSCs is costly, inefficient, and time consuming. The anti-inflammatory properties of adipose tissue-derived EVs and other biogenic nanoparticles have not been explored. In this study, biogenic nanoparticles are obtained directly from lipoaspirate, an easily accessible and abundant source of biological material. Compared to ADSC-EVs, lipoaspirate nanoparticles (Lipo-NPs) take less time to process (hours compared to months) and cost less to produce (clinical-grade cell culture facilities are not required). The physicochemical characteristics and anti-inflammatory properties of Lipo-NPs are evaluated and compared to those of patient-matched ADSC-EVs. Moreover, guanabenz loading in Lipo-NPs is evaluated for enhanced anti-inflammatory effects. Apolipoprotein E and glycerolipids are enriched in Lipo-NPs compared to ADSC-EVs. Additionally, the uptake of Lipo-NPs in hepatocytes and macrophages is higher. Lipo-NPs and ADSC-EVs have comparable protective and anti-inflammatory effects. Specifically, Lipo-NPs reduce toll-like receptor 4-induced secretion of inflammatory cytokines in macrophages. Guanabenz-loaded Lipo-NPs further suppress inflammatory pathways, suggesting that this combination therapy can have promising applications for inflammatory diseases.
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