期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 304, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.127299
关键词
Single-dye; Fluorescent nanoprobe; Ratiometric detection; Hypochlorous acid; Lysosomes
资金
- NSFC [51603067, 51773056, 21705040, 21574112]
- Hunan Provincial Natural Science Foundation of China [2018JJ3143]
- China Postdoctoral Science Foundation [2017M622571, 2018T110824]
- Open Project Program of State Key Laboratory of Chemo/Biosensing and Chemometrics [2016019, 2018011]
- Open Project Program of Key Laboratory for High Performance and Functional Polymer Materials of Guangdong Province (South China University of Technology) [20160005]
- Open Fund of the State Key Laboratory of Luminescent Materials and Devices (South China University of Technology) [2019-skllmd-09]
- National Experimental Teaching Demonstration Center of Chemical Engineering and Material (Hunan University of Science and Technology)
- Bundesministerium fur Bildung und Forschung [031A360D]
The design of novel ratiometric nanoprobes with two well-resolved fluorescence bands for accurate imaging of lysosomal hypochlorous acid (HClO) is significant to understand its biological and pathological behaviors. Herein, we develop a single-dye-doped self-referenced ratiometric nanoprobe (SSRN) for imaging of HClO in lysosomes under a single-wavelength excitation (lambda(ex)= 405 nm). The referenced unit (benzothiazole moiety) and HClO-responsive unit (rhodamine B) are integrated into the single dye (HB-Py) to achieve the self-referenced ratiometric effect. The well-dispersed SSRN are prepared by a simple coprecipitation of the morpholine moiety modified amphiphilic polymers (CO-720-MA) and HB-Py. Of note, the two well-resolved fluorescence bands (similar to 130 nm) of SSRN could provide accurate imaging of analyte in live cells. Additionally, other distinct merits with SSRN are also simultaneously realized, including high water solubility, high sensitivity (detection limit: 52 nM), excellent selectivity, good biocompatibility and excellent photostability. Significantly, benefiting from the morpholine moiety, this nanoprobe SSRN can specially locate lysosomes and enable ratiometric imaging of exogenous/endogenous HClO in lysosomes.
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