Porous CuO nanobelts assembly film for nonenzymatic electrochemical determination of glucose with High fabrication repeatability and sensing stability

Due to high sensitivity, nonenzymatic glucose sensors based on nanomaterials have attracted great attention in various fields. However, it is still a critical issue to develop a highly sensitive, selective and stable nonenzymatic glucose sensor. In this manuscript, a Langmuir-Blodgett (L-B) assembly technique along with in situ thermal oxidation has been proposed to repeatedly construct a stable nonenzymatic electrochemical glucose sensor based on porous CuO nanobelts (CuO PNBs) film as an example. Cu2Se nanobelts are first assembled to be a uniform and dense film via the L-B technique. After transferred onto indium tin oxide (ITO) electrode and followed with in situ calcination in air, they are transformed into CuO PNBs film, which is closely anchored on the ITO electrode. Through manipulating the number of assembly layer, the thickness of CuO PNBs film can be tunable. Electrochemical results demonstrate that the sensing performance of the CuO PNBs film toward glucose is highly associated with its thickness. Under the optimal thickness of about 230 nm, it exhibits the best sensitivity with a low detection limit of 60 nM in a wide linear range from 0.1 mu M to 2 mM. More importantly, the CuO PNBs assembly film displays a robust stability and anti-interference capability. Finally, the enhanced sensing mechanism and effect of the film thickness are discussed for the CuO PNBs film toward glucose.