期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 305, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.127490
关键词
Electrochemiluminescence biosensor; Carbon dots; MicroRNA detection; Catalytic hairpin assembly
资金
- National Natural Science Foundation (NNSF) of China [21904108, 21775124, 21675129]
- Natural Science Foundation Project of CQ CSTC [cstc2018jcyjAX0546]
- Chongqing Research Program of Basic Research and Frontier Technology [cstc2017jcyjAX0230]
- Fundamental Research Funds for the Central Universities [XDJK2018C041, XDJK2019TJ002]
In this work, a novel self-catalyzed carbon dots-based electrochemiluminescence (ECL) luminophore was prepared by loading the amino modified carbon dots onto the surface of AuNPs modified TiO(2)NPs (CDs-AuPEI@TiO2) and applied in sensitive ECL biosensor for miRNA-21 detection. Significantly, the prepared CDs-AuPEI@TiO2 nanocomposites exhibited strong initial ECL intensity for the acceleration of TiO(2)NPs towards to the electrochemical reduction of co-reactive H2O2, addressing the shortcomings of the strong oxidizing nature of persulfate in traditional CDs-based ECL biosensings and enhancing the detection sensitivity. Moreover, by target-induced catalytic hairpin assembly (CHA) amplification, a large number of quenching groups could be released and captured on the electrode surface, further improving the detection sensitivity. As a consequence, the ECL response of the proposed biosensor gradually decreased as the concentration of miRNA-21 increasing from 0.1 fM to 10 pM with the detection limit of 0.03 fM. The strategy provided in this work opens up a new way for miRNA detection in actual cell using self-catalyzed carbon dots-based ECL biosensing platform.
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