期刊
BIOSENSORS & BIOELECTRONICS
卷 73, 期 -, 页码 1-6出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.05.044
关键词
Colorimetric detection; Cytosensor; Dual-aptamer; Nanomaterial; Breast cancer cells
类别
资金
- National Natural Science Foundation of China [21105095, 211900040]
- State Key Instrument Developing Special Project of Ministry of Science and Technology of China [2012YQ170003]
- Instrument Developing Project of the Chinese Academy of Sciences [YZ201203]
- Natural Science Foundation of Jilin Province, China [20130101117JC]
Simple, rapid, sensitive and specific detection of cancer cells is of great importance for early and accurate cancer diagnostics and therapy. By coupling nanotechnology and dual-aptamer target binding strategies, we developed a colorimetric assay for visually detecting cancer cells with high sensitivity and specificity. The nanotechnology including high catalytic activity of PtAuNP and magnetic separation & concentration plays a vital role on the signal amplification and improvement of detection sensitivity. The color change caused by small amount of target cancer cells (10 cells/mL) can be clearly distinguished by naked eyes. The dual-aptamer target binding strategy guarantees the detection specificity that large amount of non-cancer cells and different cancer cells (10(4) cells/mL) cannot cause obvious color change. A detection limit as low as 10 cells/mL with detection linear range from 10 to 10(5) cells/mL was reached according to the experimental detections in phosphate buffer solution as well as serum sample. The developed enzymefree and cost effective calorimetric assay is simple and no need of instrument while still provides excellent sensitivity, specificity and repeatability, having potential application on point-of-care cancer diagnosis. (C) 2015 Elsevier B.V. All rights reserved.
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