期刊
BIOSENSORS & BIOELECTRONICS
卷 68, 期 -, 页码 42-48出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.12.023
关键词
Simultaneous multi-analytes detection; Graphene/gold hybrid film; Hybridization chain reaction; Biotin/streptavidin; Redox probe
类别
资金
- NNSF of China [51473136, 21275119]
- Ministry of Education of the People's Republic of China [708073]
- Natural Science Foundation of Chongqing City [CSTC-2011BA7003, CSTC-2010BB8328]
- Specialized Research Fund for the Doctoral Program of Higher Education [20100182110015]
- Fundamental Research Funds for the Central Universities [XDJK2014A012, XDJK2013A008]
- Scientific and Technological Project of CQEC [KJ130640]
A sandwich-type electrochemical immunosensor based on redox probe tags identification technology for ultrasensitive simultaneous detection of four antigens was proposed. In this project, well-distributed graphene/gold (GR-Au) hybrid film was acquired through one-step codeposition in an electrode surface and served as the base substrate for immobilizing capture antibodies (Ab1). Hybridization chain reaction (HCR) and biotin/streptavidin (B/SA), combining with gold magnetic nanoparticles were applied to increase the immobilization amount of signal tags in detection antibody (Ab2) bioconjugates. To verify this strategy, four representative biomarkers, a-fetoprotein (AFP), carcinoembryonic antigen (CEA), carbohydrate antigen (CA)125 and prostate special antigen (PSA), were used as model analytes. The resulting immunosensor could simultaneously detect four antigens in single-pass differential pulse voltammetry (DPV) scan, and exhibited obviously improved senstivity compared to previous similar immunosensors, displayed good linear relationships in the ranges from 0.2 to 800 pg/mL for AFP, 0.2 to 600 pg/mL for CEA, 0.2 to 1000 pg/mL for CAl25, 0.2 to 800 pg/mL for PSA and with detection limits of 62, 48, 77 and 60 fg/mL, respectively. (C) 2014 Elsevier B.V. All rights reserved.
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