Persistence of pesticides-based contaminants in the environment and their effective degradation using laccase-assisted biocatalytic systems

Inevitable use of pesticides due to modern agricultural practices and the associated worldwide environmental pollution has called the special attention of the researchers to overcome the persistence, recalcitrance, and multi-faceted toxicity of pesticides-based emerging contaminants. Some restricted use pesticides (RUPs) are highly toxic and carcinogenic chemicals that can be easily accumulated into non-target organisms, including humans, aquatic invertebrates, algae, and microbes. With regard to physicochemical strategies, enzymes-mediated bioremediation is a compelling and meaningful strategy for biodegradation and biotransformation of pesticides into harmless chemical species. Oxidoreductases hydrolases and transferases are among the most representative classes of enzymes pursued and engineered for this purpose. Ligninolytic enzymes, particularly laccases, are of exceptional interest due to high efficiency, specificity, eco-sustainability, and wide-ranging substrates. However, the use of native enzymes is often hindered in industrial processes for the effective removal of refractory compounds by their high cost and susceptibility. Many of these drawbacks can be addressed by enzyme immobilization on some suitable support materials. Increase in stability, reusability, reduction of product inhibition, enhanced activity, specificity, and easier product separation are amid the desirable characteristics of immobilization to construct biocatalysts for continuous systems. This review summarizes recent and up-to-date literature on the use of enzymes, explicitly, free as well as immobilized laccases in the degradation of different pesticides. In the first part, source and occurrence of pesticides in the environment, their types, and associated detrimental effects on the ecosystem/human health are comprehensively described. Afterward, we highlighted the use of different enzymes with a particular emphasis on laccase for the degradation and detoxification of an array of pesticides. Finally, the review is closed with concluding remarks, and possible future direction is proposed in this very important research arena. In conclusion, it is envisioned that effective deployment of laccase-assisted biocatalytic systems for the degradation or removal of diverse pesticides and related contaminants will help to better understand the persistence and removal fate of these hazardous pollutants. Moreover, the current research thrust presented in this review will additionally evoke researcher to engineer robust and sustainable processes to remediate pesticides-contaminated environmental matrices effectively. (C) 2019 Elsevier B.V. All rights reserved.