期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 695, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2019.133912
关键词
PM2.5; Airborne bacterial cell apoptosis; Human pathogenic bacteria; Bacterial consortia characteristic; Metabolic pathway; Biochemical function
资金
- Shandong Provincial Key Research and Development Project, China [2018GSF117004]
- Shandong Provincial Natural Science Foundation, China [ZR2016CB34]
- National Natural Science Foundation of China [41807111]
- Research Project (Youth Fund) of Shandong Academy of Sciences [2018QN0018]
Polluted air is as harmful as polluted water sources to public health. As air living organisms, the research on microbial consortia under haze stress with different PM2.5 levels in a mountainous environment remains very limited. This study investigated the dynamic changes in bacterial cell counts, apoptosis, human pathogens, consortia characteristics, metabolic pathways, and the biochemical functions under haze conditions with various degrees of pollution (leading pollutant PM2.5) from August to December 2017 in a typical mountainous terrain of Jinan, China. Samples were evaluated with flow cytometry and 16S rRNA gene amplicon sequencing. Results indicated that cell counts ranged from 6.83 x 10(5) +/- 1.27 x 10(4) (non-polluted air, NP) to 2.32 x 10(6) +/- 3.56 x 10(4) (heavily polluted air, HP) cell m(-3) air. The proportion of viable apoptotic and necrotic cells were positively correlated to PM2.5. Burkholderia cenocepacia (36.6%) was the most abundant human pathogen found in HP; this gram-negative bacterium is associated with potentially lethal respiratory infections in cystic fibrosis patients. The relative abundance of the phylum Proteobacteria (63.8%) in NP first decreased in lightly polluted (LP) (41.3%) and moderately polluted air (MP) (26.3%) then increased in HP (81.0%). Cupriavidus (22.9%) and BTEX-degrading bacteria (0.6%, Pseudomonas) were found in HP. Metabolic pathways with significant differences included cell motility and endocrine and immune diseases that exhibited increasing relative abundance as pollution levels increased. The diversity of biochemical functions was found to be decreased in hazy air. (C) 2019 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据