期刊
BIOSENSORS & BIOELECTRONICS
卷 74, 期 -, 页码 45-48出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.06.002
关键词
Organic field effect transistor; OFET; Lactate; Osmium polymer; HRP; Lactate oxidase
类别
资金
- Japan Science and Technology Agency (JST, the Center of Innovation Program)
- Japan Society for the Promotion of Science (JSPS) [26888002]
- Grants-in-Aid for Scientific Research [26888002] Funding Source: KAKEN
Biosensors based on organic field effect transistors (OFETs) are one of the more promising device applications in organic electronics. However, OFET-based biosensors are still in their early stages of development compared to other electrochemical biosensors. This study is the first to report on an extended-gate type organic field effect transistor (OFET) for lactate detection in aqueous media. Here, the extended-gate electrode of the OFET was modified with layers of a lactate oxidase and a horseradish peroxidase osmium-redox polymer on a flexible plastic film substrate for an enzymatic redox reaction of lactate. The device exhibited both high selectivity and sensitivity. The limit of detection (LOD) and the limit of quantification (LOQ) were estimated to be 66 nM and 220 nM, respectively, which are the sufficient detection limit for practical sensor applications. The obtained results confirm that extended-gate type OFET devices are applicable to enzyme-based biosensors for detecting lactate levels. (C) 2015 Elsevier B.V. All rights reserved.
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