期刊
BIOSENSORS & BIOELECTRONICS
卷 64, 期 -, 页码 554-559出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.09.079
关键词
Hemoglobin; Electrochemistry; Direct determination; Core-shell type ferromagnetic; nanoparticles; Human blood; Rat blood
类别
资金
- Polish NCN [2011/01/B/ST4/03032]
- National Centre for Research and, Development (Poland) [LIDER 527/L-4/2012]
- CePt Project [POIG.02.02.00-14-024/08-00]
An effective, fast, facile and direct electrochemical method of determination of hemoglobin (Hb) in blood sample without any sample preparation is described. The method is accomplished by using the ferromagnetic electrode modifier (carbon-encapsulated iron nanoparticles) and an external magnetic field. The successful voltammetric determination of hemoglobin is achieved in PBS buffer as well as in the whole blood sample. The obtained results show the excellent electroactivity of Hb. The measurements are of high sensitivity and good reproducibility. The detection limit is estimated to be 0.7 pM. The electrochemical determination data were compared with the gravimetric data obtained with a quartz crystal microbalance. The agreement between these results is very good. The changes of the electrode surface morphology before and after Hb detection are monitored by electron microscopy. The functionality of the electrochemical sensor is tested with human and rat blood samples. The concentration of hemoglobin in the blood samples determined by using voltammetric/gravimetric detection is in perfect agreement with the data obtained from typical clinical analysis. (C) 2014 Elsevier B.V. All rights reserved.
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