期刊
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
卷 91, 期 4, 页码 -出版社
WILEY
DOI: 10.1111/sji.12858
关键词
immune response; T cells; tuberculosis
类别
资金
- National Natural Science Foundation of China [81571961, 81772146]
- Shenzhen Science and Technology RD Grant [JCYJ20170307095244290]
- Thirteen-Fifth Mega-Scientific Project on Prevention and Treatment of AIDS, Viral Hepatitis and Other Infectious Diseases [2017ZX10201301-007-002]
- Shenzhen for Introduced High Level Medical Team of Translational Medicine of Biochip in Clinical Laboratory [SZSM201412005]
To understand functional role of PD-1-expressing MAIT cells during tuberculosis infection in humans, sorted PD-1(+) and PD-1(-) MAIT cells from pleural effusions of patients with pleural tuberculosis were subjected to transcriptome sequencing. PD-1-expressing MAIT cells were analysed by flow cytometry and their phenotypic and functional features were investigated. Transcriptome sequencing identified 144 genes that were differentially expressed between PD-1(+) and PD-1(-) MAIT cells from tuberculous pleural effusions and CXCL13 was the gene with highest fold difference. The level of PD-1-expressing MAIT cells was associated with extent of TB infection in humans. PD-1-expressing MAIT cells had increased production of CXCL13 and IL-21 as determined by flow cytometry. PD-1(high)CXCR5(-) MAIT cells were significantly expanded in pleural effusions from patients with pleural tuberculosis as compared with those from peripheral blood of both patients with tuberculosis and healthy controls. Although PD-1(high)CXCR5(-) MAIT cells from tuberculous pleural effusions had reduced IFN-gamma level and increased expression of Tim-3 and GITR, they showed activated phenotype and had higher glucose uptake and lipid content. It is concluded that PD-1-expressing MAIT cells had reduced IFN-gamma level but increased production of both CXCL13 and IL-21.
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