4.0 Article

Synthesis of Random DNA Libraries for In Vitro Selection and Analysis of Their Nucleotide Composition

期刊

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
卷 45, 期 6, 页码 656-661

出版社

MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S1068162019060141

关键词

SELEX; combinatorial libraries of nucleic acids; solid-phase synthesis; high throughput sequencing

资金

  1. Russian Scientific Foundation [16-14-10296]
  2. [AAAA-A17-117020210021-7]
  3. Russian Science Foundation [16-14-10296] Funding Source: Russian Science Foundation

向作者/读者索取更多资源

Aptamers are DNA or RNA molecules that specifically bind certain targets with high affinity due to the formation of unique spatial structures. Aptamers with specified properties could be obtained from combinatorial libraries of nucleic acids by in vitro selection procedure. Here, we optimized conditions of chemical synthesis of DNA library on the automated ASM-800 DNA/RNA synthesizer. Enzymatic hydrolysis and high-throughput sequencing methods were used to analyze compositions of synthesized combinatorial libraries. The DNA library generated under the chosen conditions is characterized by the sufficiently uniform randomization and can be applied for the in vitro selection.

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