4.5 Article

Let-7 microRNA is a critical regulator in controlling the growth and function of silk gland in the silkworm

期刊

RNA BIOLOGY
卷 17, 期 5, 页码 703-717

出版社

TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2020.1726128

关键词

Let-7 microRNA; Silk gland; CRISP; Cas9; endoreduplication; pyruvate carboxylase; glycometabolism

资金

  1. National Natural Science Foundation of China [31571334, 31071136, 31530071]
  2. National Key Basic Research Project of China [2012CB114602]
  3. Fundamental and Frontier Research Project of Chongqing [cstc2014jcyjA00025]

向作者/读者索取更多资源

The silk gland is characterized by high protein synthesis. However, the molecular mechanisms controlling silk gland growth and silk protein synthesis remain undetermined. Here we demonstrated that CRISPR/Cas9-based knockdown of let-7 or the whole cluster promoted endoreduplication and enlargement of the silk gland, accompanied by changing silk yield, whereas transgenic overexpression of let-7 led to atrophy and degeneration of the silk gland. Mechanistically, let-7 controls cell growth in the silk gland through coordinating nutrient metabolism processes and energy signalling pathways. Transgenic overexpression of pyruvate carboxylase, a novel target of let-7, resulted in enlargement of the silk glands, which is consistent with the abnormal phenotype of the let-7 knockdown. Overall, our data reveal a previously unknown miRNA-mediated regulation of silk gland growth and physiology and shed light on involvement of let-7 as a critical stabilizer and booster in carbohydrate metabolism, which may have important implications for understanding of the molecular mechanism and physiological function of specialized organs in other species.

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