期刊
REPRODUCTIVE SCIENCES
卷 27, 期 3, 页码 869-876出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s43032-019-00090-9
关键词
Endometriosis; Macrophage; IL-33
资金
- Ministry of Health, Labor and Welfare of Japan
- Ministry of Education, Culture, Sports, Science, and Technology
In endometriosis, M2 macrophages (M phi) are dominant and promote the development of endometriosis lesions. However, the factor(s) which induces M2 M phi are unknown. In the present study, we focused on interleukin (IL)-33, known as an alarmin and investigated its expression and its role in endometriosis, especially from the point of the relevance with M phi. The expression of IL-33 in endometriosis lesions was examined by immunohistochemistry. The cystic fluid of ovarian cysts/tumors was obtained and used to measure IL-33 concentration. Endometriotic stromal cells (ESC) and M phi derived from patients were used for in vitro experiments. IL-33 was detected in the epithelium and stromal cells of endometriotic lesions. The mean IL-33 concentration in the cystic fluid of endometriomas was significantly higher than that in non-endometriomas (2.2 ng/ml vs. 0.02 ng/ml, P < 0.01). IL-1 beta induced IL-33 mRNA expression in ESC via p38 MAPK activation. With IL-33 stimulation, peritoneal M phi polarized to M2 M phi and produced IL-1 beta mRNA with a 2.2-fold increase, which was negated with soluble ST2, a decoy receptor of IL-33. IL-33, derived from endometriotic lesions, stimulated M phi to produce IL-1 beta, which results in increasing IL-33 production in ESC. This cycle may continue to exacerbate the endometriotic lesions.
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