期刊
PROTEIN EXPRESSION AND PURIFICATION
卷 167, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2019.105524
关键词
Expression; Purification; GPCR; SMALP; Review
类别
资金
- BBSRC [BB/N007417/1, BB/P025927/1, BB/P022685/1, BB/M016668/1, BB/L015846/1, BB/M007529/1, BB/R02152X/1, BB/S004696/1, BB/T001488/1, 722361]
- H2020 MSCA COFUND [847419]
- H2020 ERA CoBioTech [722361]
- BBSRC [BB/S004696/1, BB/T001488/1, BB/P022685/1, BB/N007417/1, BB/P025927/1, BB/L015846/1, BB/M007529/1, BB/M016668/1, BB/R02152X/1] Funding Source: UKRI
Given their extensive role in cell signalling, GPCRs are significant drug targets; despite this, many of these receptors have limited or no available prophylaxis. Novel drug design and discovery significantly rely on structure determination, of which GPCRs are typically elusive. Progress has been made thus far to produce sufficient quantity and quality of protein for downstream analysis. As such, this review highlights the systems available for recombinant GPCR expression, with consideration of their advantages and disadvantages, as well as examples of receptors successfully expressed in these systems. Additionally, an overview is given on the use of detergents and the styrene maleic acid (SMA) co-polymer for membrane solubilisation, as well as purification techniques.
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