期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 117, 期 4, 页码 2108-2112出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1913827117
关键词
avian leukosis virus subgroup J; Na+/H+ exchanger type 1; CRISPR/Cas9 genome editing in chicken; primordial germ cells; disease resilience in poultry
资金
- Czech Science Foundation [15-23993S]
- Ministry of Education, Youth and Sports of the Czech Republic [LO1419]
- Ministry of Agriculture of the Czech Republic [QK1810344]
- Czech Academy of Sciences
Avian leukosis virus subgroup J (ALV-J) is an important concern for the poultry industry. Replication of ALV-J depends on a functional cellular receptor, the chicken Na+/H+ exchanger type 1 (chNHE1). Tryptophan residue number 38 of chNHE1 (W38) in the extracellular portion of this molecule is a critical amino acid for virus entry. We describe a CRISPR/Cas9-mediated deletion of W38 in chicken primordial germ cells and the successful production of the gene-edited birds. The resistance to ALV-J was examined both in vitro and in vivo, and the AW38 homozygous chickens tested ALV-J-resistant, in contrast to AW38 heterozygotes and wild-type birds, which were ALV-J-susceptible. Deletion of W38 did not manifest any visible side effect. Our data clearly demonstrate the antiviral resistance conferred by precise CRISPR/Cas9 gene editing in the chicken. Furthermore, our highly efficient CRISPR/Cas9 gene editing in primordial germ cells represents a substantial addition to genotechnology in the chicken, an important food source and research model.
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