期刊
BIOSENSORS & BIOELECTRONICS
卷 74, 期 -, 页码 170-176出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.06.046
关键词
Multicolor time-resolved fluorescence; Fluorescence resonance energy transfer; Aptasensor; Multiplexed Staphylococcus aureus enterotoxins; Simultaneous detection
类别
资金
- National S&T Support Program of China [2012BAK08B01]
- National Natural Science Foundation of China [21375049]
- S&T Supporting Project of Jiangsu Province [BE2012614]
- Fundamental Research Funds for the Central Universities [JUSRP51309A]
Food safety is one of the most important public health issues worldwide. Foodborne illnesses caused by Staphylococcus aureus enterotoxins (SEs) commonly occur, affecting both developing and developed countries. In this study, multicolor lanthanide-doped time-resolved fluorescence nanoparticles labeled with aptamers were used as bioprobes, and graphene oxide (GO) was employed as a resonance energy acceptor. Based on the turn down strategy, the simultaneous detection of multiplex SEs was realized in a homogeneous solution. Under the optimal conditions, the developed method exhibited high sensitivity and selectivity to three serological types of enterotoxins, including type A, B, C1, with limits of detection below 1 ng mL(-1). The application of this bioassay in milk analysis with no sample dilution was also investigated, and the results of recovery rates covered from 92.76% to 114.58%, revealing that the developed method was accurate. Therefore, this detection aptasnesor can be a good candidate for multiplex analysis and screening with simple and effective operations. (C) 2015 Elsevier B.V. All rights reserved.
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