Analysis of anthocyanin pigments in soybean hypocotyl

The T and W1 loci determine color and anthocyanin content in soybean hypocotyls. In purple hypocotyls, the anthocyanins were: A1, a delphinidin-type anthocyanin; A2 made of two unresolved peaks: peonidin 3,5-di-O-glucoside and a delphinidin-type anthocyanin; and A3, accounting for >90% of the total, was malvidin 3,5-di-O-glucoside. A single peak, peonidin 3,5-di-O-glucoside, was observed in the bronze hypocotyls.