期刊
BIOSENSORS & BIOELECTRONICS
卷 64, 期 -, 页码 511-516出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.09.064
关键词
beta-Agonist; Fluorescent bead; Immuno-chromatographic sensor; Multi-analyte detection
类别
资金
- China National ST Project [2011BAD26B0405]
- Special Fund for Agro-scientific Research in the Public Interest [201203088]
- National Natural Science Foundation of China [31201832]
- International Cooperation Program [2012DFA31140]
A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three beta-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and beta-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding beta-agonist in the conjugating zone. The unreacted antibodies were captured by beta-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 beta-agonizts in one single test without any further sample preparation. The test results can be obtained within 10 min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10 ng/mL, 0.10 ng/mL and 0.09 ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC-MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of beta-agonizts. (C) 2014 Elsevier B.V. All rights reserved.
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