期刊
PLANT JOURNAL
卷 103, 期 1, 页码 308-322出版社
WILEY
DOI: 10.1111/tpj.14732
关键词
Nicotiana attenuata; plant movement; auxin signalling; circadian clock
资金
- Max Planck Society
- ERC [293926]
- Next-Generation BioGreen 21 Program by the Rural Development Administration [PJ01322603, PJ01311601]
- Collaborative Research Centre `Chemical Mediators in Complex Biosystems-ChemBioSys' from the DFG [SFB 1127]
- Humboldt Postdoctoral Research Fellowship
- European Research Council (ERC) [293926] Funding Source: European Research Council (ERC)
Circadian organ movements are ubiquitous in plants. These rhythmic outputs are thought to be regulated by the circadian clock and auxin signalling, but the underlying mechanisms have not been clarified. Flowers of Nicotiana attenuata change their orientation during the daytime through a 140 degrees arc to balance the need for pollinators and the protection of their reproductive organs. This rhythmic trait is under the control of the circadian clock and results from bending and re-straightening movements of the pedicel, stems that connect flowers to the inflorescence. Using an explant system that allowed pedicel growth and curvature responses to be characterized with high spatial and temporal resolution, we demonstrated that this movement is organ autonomous and mediated by auxin. Changes in the growth curvature of the pedicel are accompanied by an auxin gradient and dorsiventral asymmetry in auxin-dependent transcriptional responses; application of auxin transport inhibitors influenced the normal movements of this organ. Silencing the expression of the circadian clock component ZEITLUPE (ZTL) arrested changes in the growth curvature of the pedicel and altered auxin signalling and responses. IAA19-like, an Aux/IAA transcriptional repressor that is circadian regulated and differentially expressed between opposite tissues of the pedicel, and therefore possibly involved in the regulation of changes in organ curvature, physically interacted with ZTL. Together, these results are consistent with a direct link between the circadian clock and the auxin signalling pathway in the regulation of this rhythmic floral movement.
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