期刊
BIOSENSORS & BIOELECTRONICS
卷 64, 期 -, 页码 300-305出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.09.023
关键词
Fluorescent probes; Graphene oxide; Rhodamine B; Heparin
类别
资金
- National Natural Science Foundation of China [21205002]
- Innovation Funds of Anhui Normal University [2014cxjj09]
Rhodamine B modified Polyethyleneimine (PEI) (called RB-PEI) with strong fluorescence intensity was synthesized, which contained high positively charged amine groups. The fluorescence intensity of RB-PEI can be quenched by graphene oxide (GO). The high efficiency of the quenching was caused by the non-covalent binding of RB-PEI to the GO surface by electrostatic and pi-pi interactions, forming a RB-PEI/GO complex. However, adding heparin caused intense fluorescence to be recovered, and this was caused by the anionic heparin removing the RB-PEI from the GO surface, forming a fluorescent RB-PEI/heparin complex. Under optimal conditions, the change in the fluorescence intensity when heparin was added was proportional to the heparin concentration over the range 0.09-0.9 U mL(-1). The detection limit for heparin was 0.00132 U mL(-1), which was lower than that has been achieved using other methods. This approach offers a new quantitative method for determining heparin. (C) 2014 Elsevier B.V. All rights reserved.
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