期刊
BIOSENSORS & BIOELECTRONICS
卷 64, 期 -, 页码 94-101出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.08.053
关键词
Procainamide hydrochloride; MIP; O-MSSQ; TiO2; Composite sol
类别
资金
- National Major Scientific Instrument and Equipment Development Project [2011YQ12003505]
- National Natural Science Foundation of China [21075029, 21375033]
- Wuhan City Youth Science and Technology Morning Program [2005ABA199]
In this study, we designed a novel molecularly imprinted polymer (MIP), oligomeric methyl silsesquioxane (O-MSSQ)-TiO2 composite sal, which was made using a sol-gel reaction. This polymer has structural rigidity and high surface area of O-MSSQ as well as high bio-compatibility and relatively good conductivity of the TiO2. Next, a sensitive and selective imprinted electrochemical sensor was successfully constructed for the direct detection of procainamide hydrochloride by molecularly imprinting a film onto the surface of a glassy carbon electrode. Adding TiO2 resulted in a noticeable enhancement in the sensitivity of the MIP sensor. The performance of the O-MSSQ-TiO2 film was discussed, and the optimal conditions for detection were determined. The oxidative peak current increased linearly with the concentration of procainamide hydrochloride in the range of 4.00 x 10(-9)-4.97 x 10(-5) M using differential pulse voltammetry, and the detection limit was 1.30 x 10(-9) M with S/N=3. Furthermore, the sensor was applied to determine the procainamide hydrochloride content in a human blood serum sample. The recoveries of the sensors varied from 96.77% to 101.35%, indicating that the prepared sensor might be promising for the determination of procainamide hydrochloride in clinical tests. Moreover, the imprinted electrochemical sensor was used to selectively detect procainarnide hydrochloride. The analytical application was conducted successfully and yielded accurate and precise results. (C) 2014 Elsevier B.V. All rights reserved.
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