期刊
CANADIAN JOURNAL OF NEUROLOGICAL SCIENCES
卷 44, 期 1, 页码 90-95出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/cjn.2016.284
关键词
oxidative stress; ALS; biomarkers; neuroinflammation; homocysteine; vitamin
Background: Pathophysiological mechanisms that contribute to neurodegeneration in Amyotrophic Lateral Sclerosis (ALS) include oxidative stress and inflammation. We conducted a preliminary study to explore these mechanisms, to discuss their link in ALS, and to determine the feasibility of incorporating this combined analysis into current biomarkers research. Methods: We enrolled 10 ALS patients and 10 controls. We measured the activities of glutathione peroxidase, glutathione reductase, superoxyde dismutase (SOD), and the levels of serum total antioxidant status (TAS), malondialdehyde (MDA), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and glutathione status (e.g. glutathione disulfide, GSSG/reduced glutathione, GSH). We analysed the concentrations of homocysteine, several cytokines, vitamins and metals by standard methods used in routine practice. Results: There was a significant decrease in TAS levels (p = 0.027) and increase in 8-OHdG (p = 0.014) and MDA (p = 0.011) levels in ALS patients. We also observed a significantly higher GSSG/GSH ratio (p = 0.022), and IL-6 (p = 0.0079) and IL-8 (p = 0.009) concentrations in ALS patients. Correlations were found between biological and clinical markers (homosysteine vs. clinical status at diagnosis, p = 0.02) and between some biological markers such as IL-6 vs. GSSG/GSH (p = 0.045) or SOD activity (p = 0.017). Conclusion: We confirmed the systemic alteration of both the redox and the inflammation status in ALS patients, and we observed a link with some clinical parameters. These promising results encourage us to pursue this study with collection of combined oxidative stress and inflammatory markers.
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