期刊
BIOSENSORS & BIOELECTRONICS
卷 74, 期 -, 页码 454-460出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.06.080
关键词
Electroless plating; Optical fiber SPR biosensor; Polydopamine; Surface functionalization; Antibody immobilization; Immunoassays
类别
资金
- Ministry of Science and Technology of China [2012YQ090194, 2012AA06A303, 2012BAD29B05]
- National Natural Science Foundation of China [51473115, 21276192]
- Ministry of Education of China [B06006, NCET-11-0372]
A sensitive and stable electroless-plated gold film for the preparation of an optical fiber surface plasmon resonance (SPR) sensor is presented in this work, together with a facile antibody immobilization method. Gold nanoparticles were uniformly adsorbed onto the surface of an optical fiber forming a film with a thickness of approximately 563 nm. The sensor had a high sensitivity with 2054 nm/RIU and 3980 nm/RIU in the refractive index ranges of 1.333-1.359 and 1.359-1386, respectively. An SPR biosensor was developed based on polydopamine-modified gold film (PDA-Au), which was fabricated by a simple and quick spontaneous polymerization of dopamine (DA) on the gold film. When goat anti-human IgG antibodies were immobilized, the FDA-Au surface had a larger resonant wavelength shift of 66.21 nm compared with the traditional 11-mercaptoundecanoic acid-modified gold film (MUA-Au) surface. In addition, the PDA-Au surface enabled the sensitive and selective determination of human IgG down to a concentration of 2 mu g mL(-1) with a high sensitivity of 0.41 nm per mu g mL(-1). The FDA-Au surface exhibited an approximately four fold higher sensitivity and an about seven fold lower LOD than the MUA-Au surface to human IgG. (C) 2015 Elsevier B.V. All rights reserved.
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